A Portable Biosensing Device with Magnetic Separation and Quantum Dot Bead Labeling for Simple, Rapid, and Quantitative Detection of Salmonella Typhimurium
X. Xi, R. Wang, P. Yao, L. Yao, S. Tung, Y. Li
Published in Transactions of the ASABE 63(6): 1947-1955 (doi: 10.13031/trans.13880).
Copyright 2020 American Society of Agricultural and Biological Engineers.
The authors are Xinge Xi, Graduate Research Assistant, and Ronghui Wang, Research Scientist, Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, Arkansas; Ping Yao, Assistant Professor, College of Information and Electrical Engineering, Shenyang Agricultural University, Shenyang, China; Lan Yao, Graduate Student, Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture, China Agricultural University, Beijing, China; Steve Tung, Professor, Department of Mechanical Engineering, and Yanbin Li, Distinguished Professor, Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, Arkansas. Corresponding author: Yanbin Li, 230 Engineering Hall, University of Arkansas, Fayetteville, AR 72701; phone: 479-575-2881; e-mail: yanbinli@uark.edu.
A portable biosensing device was designed, fabricated, and evaluated for Salmonella detection.
A mixing, separation, and detection chamber (MSDC) was developed and used for Salmonella detection.
The disposable MSDC prevented cross-contamination and reduced costs.
The device was mostly automated for Salmonella detection and feasible for in-field applications.
Abstract. Foodborne pathogenic bacteria have caused numerous illnesses and economic losses in the U.S. and the world. It is highly important for food industries to conduct in-field screening for pathogenic bacteria to ensure food safety. The objective of this study was to convert our previously developed optical biosensing method into a portable biosensing device to achieve simple, rapid, and quantitative detection of Salmonella Typhimurium. The device consisted of a control module, a magnetic separation module, and a fluorescence detection module. The bacteria sample, immuno-magnetic nanoparticles, and immuno-quantum dot beads were added into a mixing, separation, and detection chamber and were fully mixed in the control module. The sample was then moved to the magnetic separation module to automatically separate and concentrate the target bacteria. Finally, the fluorescence intensity was measured with the fluorescence detection module to determine the concentration of the target bacteria. The device was able to perform separation and detection of Salmonella with minimal manual operation with a detection limit of 5.4 x 102 CFU mL-1 in 1 h. The device also showed good specificity against four selected non-target bacteria.